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. 1998 Aug 18;95(17):9950–9955. doi: 10.1073/pnas.95.17.9950

Figure 4.

Figure 4

Exogenous insulin increases Hsc70 mRNA and protein levels in cultured embryos. (A) Northern blot analysis of hsc70 mRNA in whole chicken embryos cultured in basal medium (M) or in the presence of insulin (Ins) for 12 h. The single 2.2-kb transcript was quantified relative to methylene blue-stained 18S rRNA (Lower). The relative densitometric values (mean of two blots) were 1 (M), 1.3 (10−8 M insulin) and 1.2 (10−7 M insulin). (B) Extracts of embryos cultured in basal medium or in the presence of insulin for 12 h were subjected to SDS-PAGE and immunoblotting. The staining with antiserum anti-Hsc70pep revealed a 73-kDa band that was quantified relative to tubulin (Lower). The relative Hsc70 densitometric values (mean of four blots) were 1 (M), 1.16 (10−9 M insulin), 1.25 (10−8 insulin).