Figure 5.

Binding of LrpA to the lat upstream region shown by the electrophoretic mobility shift assay (EMSA). (A) Gel shift was performed with (15 fmol) 300-bp fragment (−300 to +1) encompassing the promoter and transcription initiation site of the lat gene. Lanes 1 and 2: 50 and 100 ng of LrpA, respectively; lane 3: without LrpA (control). (B) The assay was performed with 100 ng of purified M. tuberculosis LrpA and the 23-bp fragment (15 fmol) in the presence and absence of 10 mM or 20 mM leucine. Lane 1: 100 ng of LrpA with no leucine; lane 2: 100 ng of LrpA with 10 mM leucine; lane 3: 100 ng LrpA with 20 mM leucine.