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Gel shift assay of the C domain in Ph1280p. ssRNA (A) and dsRNA (B) used for the gel shift assay were prepared as described in Materials and Methods. 32P-labeled ssRNA or dsRNA were incubated with the indicated concentrations of the C domain and separated on a 20% polyacrylamide gel that was run at 4°C. *–, free ssRNA; *=, free dsRNA.
