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. 2000 May;9(5):1035–1037. doi: 10.1110/ps.9.5.1035

Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein.

S J Blance 1, N L Williams 1, Z A Preston 1, J Bishara 1, M S Smyth 1, A Maxwell 1
PMCID: PMC2144630  PMID: 10850814

Abstract

Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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