Analysis of the mutational disruption of the ScNM and CaNM recognition elements. a, Quantification of the relative affinity of the full-length Sc/Ca NM chimera for wild-type ScNM peptides (dark blue bars) and ScNM peptides containing the S17R mutation (yellow bars). b, Quantification of the relative affinity of the full-length Sc/Ca NM chimera for wild-type CaNM peptides (dark blue bars) and CaNM peptides containing the G70, 71, 80, 81A mutations (pink bars). The peptide arrays were incubated with the NM chimera (1 μM NM, 5% Alexa Fluor 647) for two hours.