Fig. 3.
Elovl4 mRNA expression in eyecups from 1-month and 1-year-old wt and Stgd3-heterozygous mice. S1-nuclease protection assay detects a 182-N fragment of a 322-N probe protected by wt Elovl4 mRNA, and two fragments (126 Ns and 51 Ns) protected by mutated Elovl4 (Stgd3) mRNA. The molar amount of Elovl4 mRNA in wt mouse eyecups is equal to the combined molar amounts of wt and Stgd3 mRNAs in Stgd3-heterozygous eyecups.