Figure 4.

MMS killing after induction of the GAL1 promoter with galactose (A) or repression with glucose (B) for the Mag⁺ wild-type yeast strain BGY111 containing the control plasmid pYES (○) or its isogenic mag1Δ derivative strain BGY148 containing the plasmid pYES (•), pYES-tag (▿), pYES-MAG (▾) or pYES-AAG (□). A representative experiment is shown in each case; the relative survival between the various strains was always consistent. In addition, for the galactose-induced cells, qualitatively similar results were obtained in apn1Δ (BGY113) and apn1Δ mag1Δ (BGY149) strain backgrounds (data not shown). (C) MMS killing after galactose induction for the Mag⁺ wild-type strain BGY111 containing the control plasmid pYES (○) or its isogenic apn1Δ derivative strain BGY113 containing the plasmids pYES(•), pYES-MAG (▿), YEpAPN1 (▾), or pYES-MAG and YEpAPN1 (□).