Table 3.
Effect of mutations on the frequency of tract alteration in (AC)n and (TG)n inserts
| Relevant genotype | (AC)18 | (AC)51 | (TG)21 | (TG)42 |
|---|---|---|---|---|
| Wild type | 5.9 × 10−6 (1.0) | 2.2 × 10−4 (1.0) | 2.2 × 10−5 (1.0) | 1.9 × 10−4 (1.0) |
| recA | 2.5 × 10−5 (4.2) | 1.9 × 10−4 (0.9) | 4.4 × 10−5 (2.0) | 1.9 × 10−4 (1.0) |
| sbcD | 8.1 × 10−6 (1.4) | 2.3 × 10−4 (1.0) | 7.4 × 10−6 (0.3) | 2.8 × 10−4 (1.5) |
| rep | nd | 8.3 × 10−4 (3.8) | 6.7 × 10−5 (3.0) | 8.3 × 10−4 (4.4) |
| polA1 | 1.3 × 10−4 (22) | 8.7 × 10−4 (4.0) | 1.9 × 10−4 (8.6) | 6.5 × 10−4 (3.4) |
| ΔpolA (F′ Klenow) | 1.8 × 10−4 (30) | 2.7 × 10−3 (13) | 3.7 × 10−4 (17) | 3.3 × 10−3 (17) |
| uvrD | nd | 3.2 × 10−3 (15) | 5.3 × 10−4 (24) | 3.7 × 10−3 (19) |
| mutS | 5.7 × 10−4 (97) | 5.9 × 10−3 (27) | 9.6 × 10−4 (44) | 3.0 × 10−2 (158) |
| mutD | (≥1,000) | (≥1,000) | (≥1,000) | (≥1,000) |
| dam | nd | 2.8 × 10−3 (13) | 8.5 × 10−4 (39) | 3.4 × 10−3 (18) |
| lexA (Def) | 1.3 × 10−4 (22) | 1.7 × 10−3 (7.7) | 2.4 × 10−4 (11) | 1.7 × 10−3 (8.8) |
| lexA (Def) recA730 | nd | 1.5 × 10−3 (6.8) | nd | nd |
Frequencies of tract alteration represent the average of at least three independent determinations. Numbers in parentheses represent the rates of tract length alteration (fold) relative to the rate in the wild-type strain for each insert. nd, not determined.