Figure 1.

RNA gel blot analysis of mitochondrial transcripts in test-cross or backcross populations segregating for male sterility and sterility restoration. For the top two panels, mtRNA samples from cross I progeny (Table 2) were probed with the Brassica atp6 (top panel) or nad4 (second panel) coding sequences to follow the segregation of Rfp and Mmt, respectively. For the bottom two panels, mtRNA samples from cross III progeny (Table 2) were probed with the nad4 (top panel) or orf139 (bottom panel) coding sequences. The sizes of transcripts specific to Rfp (mmt) plants (top panel) or Rfn (Mmt) plants (bottom three panels) are indicated to the right of the autoradiograms.