Table 2.
Genetic crosses used to assess allelism relationships among rfp, Rfn, and Mmt and the map position of Rfn
| Cross | No. of plants
|
Ratio |
Mmt-specific 1.6-kb nad4 transcript detected in
|
||
|---|---|---|---|---|---|
| Fertile | Sterile | Fertile, % | Sterile, % | ||
| pol cytoplasm | |||||
| I. Westar (pol) Rfp, mmt/rfp, Mmt × Bronowski (cam) rf, mmt/rf, mmt | 39 | 36 | 1:1 | 0 | 100 |
| Nap cytoplasm | |||||
| II. nap CMS* × Westar (pol) Rfp, mmt/rfp, Mmt | 4 | 3 | 1:1 | 100 | 0 |
| III. BC1: nap CMS × a fertile progeny from cross II | 16 | 14 | 1:1 | 100 | 0 |
| IV. BC2: nap CMS × a fertile progeny from cross III | 17 | 17 | 1:1 | 100 | 0 |
| V. BC2: nap CMS × [nap CMS × (nap CMS × Karat†)] | 4 | 5 | 1:1 | 100 | 0 |
| VI. BC1: nap CMS × (nap CMS × Karat) | 18 | 10 | 1:1‡ | ND | ND |
ND, not determined.
*
nap CMS: male sterile Brownowski (nap) rf, mmt/rf, mmt.
†
Genotype of the variety Karat: Rfn, rfp, Mmt/Rfn, rfp, Mmt (nap cytoplasm).
‡
Deviance from the expected 1:1 ratio is not statistically significant (χ2 = 1.725).