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. 1998 Aug 18;95(17):10055–10060. doi: 10.1073/pnas.95.17.10055

Figure 1.

Figure 1

GAD65 transgenic construct and Southern blot of tail DNA. (A) Map of the transgene, comprising the enhancer and promoter regions of the MHC class I Kb gene, ligated 5′ of the murine GAD65 coding region, ligated 5′ of a series of noncoding exons from the HGH gene. The introduced mutations replaced the pyridoxal phosphate binding motif NPHK with NPLA. The black bar represents the 700-bp probe used on Southern blots to detect a predicted 2-kb BglII fragment. (B) Southern analysis of BglII-digested genomic DNA extracted from mouse tails: lanes 1, 3, 4, 5, and 10 are tg(+) line 14; lanes 2, 6, 7, 8, and 9 are non-tg(+) littermates. The 7-kb doublet and 2-kb band correspond to endogenous and transgenic bands, respectively. Tg(+) mice of lines 12 and 13 gave essentially the same results as those shown for line 14.