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. 1998 Aug 18;95(17):10055–10060. doi: 10.1073/pnas.95.17.10055

Figure 2.

Figure 2

Transgenic mRNA and protein expression. (A) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). (B) Reverse transcription–PCR analysis of tissues from tg(+) mice (+) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian myeloblastosis virus reverse transcriptase (AMV-RT) by using transgene-specific primers (Upper) and hypoxanthine-guanine phosphoribosyltransferase-specific primers (Lower). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult (C and D) or day 2 (E) tg(+) mice (+) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 (C and E). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 (D). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).