Figure 3.

(A) Cell surface expression of Fas is restored in 293 transfectants with mutant 10.4K and 14.5K ORFs. Cells were stained with FITC-labeled goat anti-mouse IgG (thin line), the HLA specific mAb W6/32 (bold line) and the Fas specific mAb B-G27 (filled histogram). At least five independent experiments with at least three clones from each transfection were performed. In this representative experiment the mutant cell clones 293E3–10.4*-2, 293E3–14.5*-8 and 293E3–14.7*-10 were analyzed. (B) Modulation of cell surface proteins upon infection with Ad2. A549 cells were infected with 50 multiplicity of infection per cell of Ad2 for 32 h or were mock treated. The cell surface proteins analyzed are given in the histogram. As control, cells were incubated with fluorescein isothiocyanate-labeled goat anti-mouse IgG. Staining of mock-treated cells is indicated by thin lines, that of infected cells by bold lines.