Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1986 May;166(2):541–544. doi: 10.1128/jb.166.2.541-544.1986

Isolation of ntrA-like mutants of Azotobacter vinelandii.

E Santero, F Luque, J R Medina, M Tortolero
PMCID: PMC214638  PMID: 3009406

Abstract

A number of chlorate-resistant mutants of Azotobacter vinelandii affected in a general control of nitrogen metabolism were isolated. These mutants could not utilize dinitrogen, nitrate, or nitrite as a nitrogen source. The reason for this inability is that they were simultaneously deficient in nitrogenase and nitrate and nitrite reductase activities. They were complemented by a cosmid carrying a DNA fragment of A. vinelandii able to complement ntrA mutants of Escherichia coli, so they seemed to be ntrA-like mutants.

Full text

PDF
541

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bishop P. E., Brill W. J. Genetic analysis of Azotobacter vinelandii mutant strains unable to fix nitrogen. J Bacteriol. 1977 May;130(2):954–956. doi: 10.1128/jb.130.2.954-956.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Guerrero M. G., Vega J. M., Leadbetter E., Losada M. Preparation and characterization of a soluble nitrate reductase from Azotobacter chroococcum. Arch Mikrobiol. 1973 Jun 25;91(4):287–304. doi: 10.1007/BF00425049. [DOI] [PubMed] [Google Scholar]
  3. Kennedy C., Robson R. L. Activation of nif gene expression in Azotobacter by the nifA gene product of Klebsiella pneumoniae. Nature. 1983 Feb 17;301(5901):626–628. doi: 10.1038/301626a0. [DOI] [PubMed] [Google Scholar]
  4. Kleiner D. Ammonium uptake by nitrogen fixing bacteria I. Azotobacter vinelandii. Arch Microbiol. 1975 Jun 22;104(2):163–169. doi: 10.1007/BF00447319. [DOI] [PubMed] [Google Scholar]
  5. LOWE R. H., EVANS H. J. PREPARATION AND SOME PROPERTIES OF A SOLUBLE NITRATE REDUCTASE FROM RHIZOBIUM JAPONICUM. Biochim Biophys Acta. 1964 Jun 1;85:377–389. doi: 10.1016/0926-6569(64)90301-3. [DOI] [PubMed] [Google Scholar]
  6. Lepo J. E., Wyss O., Tabita F. R. Regulation and biochemical characterization of the glutamine synthetase of azotobacter vinelandii. Biochim Biophys Acta. 1982 Jun 24;704(3):414–421. doi: 10.1016/0167-4838(82)90062-0. [DOI] [PubMed] [Google Scholar]
  7. Magasanik B. Genetic control of nitrogen assimilation in bacteria. Annu Rev Genet. 1982;16:135–168. doi: 10.1146/annurev.ge.16.120182.001031. [DOI] [PubMed] [Google Scholar]
  8. Markwell M. A., Haas S. M., Bieber L. L., Tolbert N. E. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978 Jun 15;87(1):206–210. doi: 10.1016/0003-2697(78)90586-9. [DOI] [PubMed] [Google Scholar]
  9. Ow D. W., Ausubel F. M. Regulation of nitrogen metabolism genes by nifA gene product in Klebsiella pneumoniae. Nature. 1983 Jan 27;301(5898):307–313. doi: 10.1038/301307a0. [DOI] [PubMed] [Google Scholar]
  10. Page W. J., von Tigerstrom M. Optimal conditions for transformation of Azotobacter vinelandii. J Bacteriol. 1979 Sep;139(3):1058–1061. doi: 10.1128/jb.139.3.1058-1061.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Payne W. J. Reduction of nitrogenous oxides by microorganisms. Bacteriol Rev. 1973 Dec;37(4):409–452. doi: 10.1128/br.37.4.409-452.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Piéchaud M., Puig J., Pichinoty F., Azoulay E., Le Minor L. Mutations affectant la nitrate-réductase A et d'autres enzymes bactériennes d'oxydoréduction. Ann Inst Pasteur (Paris) 1967 Jan;112(1):24–37. [PubMed] [Google Scholar]
  13. Roberts G. P., Brill W. J. Genetics and regulation of nitrogen fixation. Annu Rev Microbiol. 1981;35:207–235. doi: 10.1146/annurev.mi.35.100181.001231. [DOI] [PubMed] [Google Scholar]
  14. Ruvkun G. B., Ausubel F. M. A general method for site-directed mutagenesis in prokaryotes. Nature. 1981 Jan 1;289(5793):85–88. doi: 10.1038/289085a0. [DOI] [PubMed] [Google Scholar]
  15. Sadoff H. L., Shimel B., Ellis S. Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes. J Bacteriol. 1979 Jun;138(3):871–877. doi: 10.1128/jb.138.3.871-877.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sibold L., Elmerich C. Constitutive expression of nitrogen fixation (nif) genes of Klebsiella pneumoniae due to a DNA duplication. EMBO J. 1982;1(12):1551–1558. doi: 10.1002/j.1460-2075.1982.tb01354.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sorger G. J. Regulation of nitrogen fixation in Azotobacter vinelandii OP: the role of nitrate reductase. J Bacteriol. 1969 Apr;98(1):56–61. doi: 10.1128/jb.98.1.56-61.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stewart W. D., Fitzgerald G. P., Burris R. H. In situ studies on N2 fixation using the acetylene reduction technique. Proc Natl Acad Sci U S A. 1967 Nov;58(5):2071–2078. doi: 10.1073/pnas.58.5.2071. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Vega J. M., Guerrero M. G., Leadbetter E., Losada M. Reduced nicotinamide-adenine dinucleotide-nitrite reductase from Azotobacter chroococcum. Biochem J. 1973 Aug;133(4):701–708. doi: 10.1042/bj1330701. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES