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. 1998 Aug 18;95(17):10094–10099. doi: 10.1073/pnas.95.17.10094

Figure 5.

Figure 5

T cell recognition of allogeneic MHC class II is not restricted to a single MHC-peptide complex and can be blocked by conformation-sensitive antibodies. (A) Proliferation of a polyclonal T cell line derived from in vitro immunization of bm12 CD4 T cells with Ab+, Ii− splenocytes. Significant proportion of cells reacts to L cells expressing Ab-mCLIP. (B) Recognition pattern of hybridomas (bars 3 and 18 serve as examples) derived from the T cell line shown in A is similar to the allorecognition pattern of D10. Proliferation of IL-2 sensitive CTLL line was measured by Alamar blue colorimetric assay. Results are expressed as arbitrary OD units at 570 nm vs. 600 nm. (C) Conformation-sensitive mAb 25-9-17 blocks the reactivities of the two hybridomas to both Ab+, Ii− splenocytes and Ab-mCLIP covalent complex expressed by Ii− L cell transfectants.