Table 1.

PBL of MVA-SIV_SM gag pol-vaccinated Mamu-A^*01+ rhesus monkeys demonstrate binding to tetrameric Mamu-A^*01/p11C, C-M complex

Immunization	Monkey	Assay	Day post-immunization
			Primary			Secondary
			14	35	91	101	119
MVA-SIVSMgag pol	442	Fresh*	0	0	0	0.7	0.3
		Peptide-stimulated†	NT§	NT	11.3	21.5	25.9
		% Specific lysis‡	50.0	24.0	27.0	42.0	45.0
	443	Fresh	0.1	0.3	0.1	4.9	0.7
		Peptide-stimulated	NT	NT	13.3	23.9	27.1
		% Specific lysis	33.0	33.0	31.0	43.0	59.0
	446	Fresh	0	0	0	0.6	0.6
		Peptide-stimulated	NT	NT	13.4	44.4	35.7
		% Specific lysis	3.0	26.0	24.0	54.0	41.0
	449	Fresh	0	0	0	1.7	0.5
		Peptide-stimulated	NT	NT	5.6	18.5	15.6
		% Specific lysis	1.0	0	1.0	40.0	35.0
Control MVA	454	Fresh	0	0	0	0	0
		Peptide-stimulated	NT	NT	0	0	0
		% Specific lysis	1.0	1.0	0	0	0
	459	Fresh	0	0	0	0	0
		Peptide-stimulated	NT	NT		0
		% Specific lysis	3.0	0	0	0	0

^*Lymphocytes were assessed for binding of tetramer to CD8αβ+ T cells in whole blood. 

^†PBLs were cultured with p11C and then tetramer binding to CD8αβ+ T cells was assessed. 

^‡PBLs were cultured with p11C and 7 days later p11C, C-M-specific lysis was determined in a standard ⁵¹Cr-release assay. Data represent % specific lysis at an E:T ratio of 2.5:1. 

^§NT, not tested.