Table 2.
Immunization | Monkey | % CD8αβ tetramer binding
|
||
---|---|---|---|---|
Fresh* | Peptidestimulated† | % Specific lysis‡ | ||
MVA-SIVSMgag pol | 442 | 0.1 | 10.7 | 24 |
443 | 0.5 | 15.8 | 26 | |
446 | 0.3 | 11.6 | 20 | |
449 | 0.3 | 13.0 | 28 | |
MVA control | 454 | 0.0 | 0.0 | 0 |
459 | 0.0 | 0.0 | 0 |
Lymph node lymphocytes were assessed for binding of tetramer to CD8αβ+ T cells.
Lymph node cells were cultured with p11C and then tetramer binding to CD8αβ+ T cells were assessed.
Lymph node cells were cultured with p11C and 7 days later p11C, C-M-specific lysis was determined in a standard 51Cr-release assay. Data represent % specific lysis at an E:T ratio of 2.5:1.