Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers for Wlds and β-tubulin have very similar R2 values. Standard curves for the Wlds and β-tubulin gene in a serially diluted (1 in 10) template genomic DNA sample from wild-type (C57Bl/6J). Efficient amplification was obtained for both sets of primers, as demonstrated by the slopes of linear regression of the standard curves, and good correlation coefficients. Ct is cycle threshold.
