Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2007 Oct 30;2:21. doi: 10.1186/1750-1326-2-21

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2007 Wishart et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

QPCR can be used to determine copy number in transgenic animals. QPCR carried out on samples of genomic DNA can be used to determine copy number of an insert in rat transgenic lines (Tg) 23 and 79. Although there is some fluctuation in the determined copy number, wild-type (WT) animals are expected to have 2 copies. Heterozygous Wld^s(Het-Wld) are expected to have 4 copies and homozygous Wld^s(Hom-Wld) should have 6 copies. Analysis of the QPCR data showed the following copy numbers: WT 1.15 ± 0.68 (mean ± SEM) copies (N = 13), Het-Wld 3.87 ± 0.36 copies (N = 7), Hom-Wld 6.10 ± 0.53 copies (N = 7), Tg23 12.34 ± 2.05 (N = 10) and Tg79 19.49 ± 1.52 (N = 14).