Figure 2.

Infectivity of the 16PVs that have bound to BPEOIA, NEM, DTNB, MBTA, or MTSET. The 16PVs were incubated with the thiol-reactive reagent indicated at 37°C for 2 h. The samples were diluted by 1000-fold and added to HeLa cells. The cells were incubated for 2 days and harvested. The cells expressing EGFP were counted by a FACS. BPEOIA: biotin polyethyleneoxide iodoacetamid, NEM: N-ethylmaleimide, DTNB: 5,5'-dithiobis(2-nitrobenzoic acid), MBTA: 4-(N-maleimido)benzyl-trimethylammonium iodide, MTSET: [2-(trimethylammonium)ethyl] methanethiosulfonate bromide.