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Binding of BPEOIA to L1 of the 16PV. (A) The 16PVs were incubated with 1 mM biotin-PEO-iodoacetamide (1 mM) in DMEM at 37°C for 2 h, electrophoresed on an SDS-polyacrylamide gel, and stained with SYPRO Ruby. (B) The proteins in the gel were transferred to a polyvinylidene difluoride membrane and probed with Streptavidin-HRP.
