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Sedimentation and morphology of the 16PVs that have bound to DTNB or NEM. (A) The 16PVs were incubated with DTNB (2 mM) or NEM (2 mM) at 37°C for 2 h. The sample was loaded on the top of a linear sucrose-density gradient (5 to 40%) and centrifuged. L1 in the fractions obtained by a bottom puncture was detected by immunoblotting with mouse anti-HPV16L1 antibody. (B) The 16PVs were incubated with NEM (2 mM) at 37°C for 2 h and observed under a transmission electron microscope.
