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. 2008 Jan 2;3(1):e1375. doi: 10.1371/journal.pone.0001375

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Hao et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Yeast two-hybrid assay of the interaction between YopJ or the YopJ(C172A) mutant and wild type Pbs2 or mutants of Pbs2 in or around G α-helix. (B) Recombinant YopJ is incubated with purified Pbs2 kinase domain or mutants in or around the G α-helix in the presence of ¹⁴C-labeled acetyl-CoA for 1 hour at 30°C. Samples are separated by SDS-PAGE and analyzed by autoradiography. Substrate loading was controlled by protein staining. (C) Yeast two-hybrid assay of the interaction between YopJ or YopJ (C172A) mutant and corresponding MKK1 mutants. (D) Structural mapping of G α-helix and APE domain, showing the mutants tested above.