Figure 5. Adoptive transfer of SPC and purified T cells from N-4YSyn vaccinated B10.BR donors leads to infiltration of T cells in the SNpc of MPTP mice on day 2.

(A) Frequency of CD3⁺ T cells and CD19⁺ B cells before and after enrichment of T cells. Population of enriched T-cells was 94% CD3⁺ prior to adoptive transfer to B10.BR mice. (B) Sections throughout the SNpc were immunostained for CD3 and counterstained with thionin. Clusters of CD3⁺ cells are observed within the SNpc (arrowheads) as seen at 100× magnification (left). Magnification (600X) of boxed area (left panel) is shown (right panel). CD3⁺ cells are small and round exhibiting lymphocyte morphology.