Table 1. Wild-type C1845-induced gene expression.

Genes (assay ID)	Fold induction
VEGF (Hs00173626_m1)	3.60±0.45 (n = 4)*
IL8 (Hs00174103_m1)	1.70±0.16 (n = 3)*
PlGF (Hs00182176_m1)	1.00±0.16 (n = 3)*
TGFβ R2 (Hs00234253_m1)	0.78±0.21 (n = 2)
EGF R1 (Hs00193306_m1)	0.70 (n = 2)

Cells were infected for four hours with Afa/Dr DAEC wild-type strain C1845 and total RNA extracted. Selected gene expression was assayed by q-PCR. The oligonucleotides used in this study were designed by Applied Biosystems, the manufacturer's references are given in brackets. Abbreviations are as follows: VEGF: Vascular Endothelial Growth Factor, IL8: interleukin 8, PlGF: Placental growth factor, TGFβ R2: Transforming growth factor Receptor 2, EGF R1: Epithelial growth factor Receptor 1. Results (means±SD) are representative of independent experiments (where n represents the number of experiments). *, p<0.001 versus non infected cells.