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Increased antigen specificity in CD4+ cells expressing MART-HM. Purified CD4+ human PBLs expressing either the MART-HH (white columns) or the MART-HM (black columns) were cocultured with the HLA-A2+/melanoma cell lines 526 and 624, the HLA-A2−/melanoma 938, or without target (NT). The concentration of IFN-γ (A), GM-CSF (B), and IL-2 (C) secreted in the medium was measured using an ELISA procedure.
