Figure 3.

Enrichment of H/RS cells from a HDns affected lymph node (patient 2428). (Upper) Two populations of CD30⁺ cells are enriched, which are stained differentially bright for this antigen. (Lower) Counterstaining of the enriched cells with CD15-phycoerythrin. Only the CD30-brightly-stained cells are also positive for CD15. The scatter characteristics of the two CD30-positive populations were analyzed by using electronic gating. Only the CD30-bright cells that are also CD15⁺ display the characteristics of very big and granulated cells. The CD30-dim/CD15⁻ cells show scatter characteristics of small lymphocytes.