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Slot blot analysis of DP3 products. Plasmid DNA was denatured and transferred to duplicate nylon membranes. The blots were probed with equal cpm/ml of radiolabeled EML or EPRO cDNA. These are representative blots containing subcloned DP3 fragments from the first round of RDA. pBluescript and β-actin were included on the blot as negative and positive controls, respectively.
