Table 2. Average Foliar Glucosinolate Content in plants containing the 35S:MYB29 or 35S:MYB76 transgene.
Trait | Col-0 | MYB29 | MYB76 | |||||
N = 19 | N = 31 | N = 24 | ||||||
Mean | SE | Mean | SE | Sig | Mean | SE | Sig | |
3MSP | 19 | 1.7 | 35.9 | 3.9 | ** | 43.5 | 3.7 | ** |
4MTB | 42.9 | 6.5 | 37.6 | 5.8 | ** | 28.6 | 5.7 | ** |
4MSB | 141.7 | 10 | 314.7 | 34.2 | ** | 366.8 | 31.5 | ** |
5MSP | 5.9 | 0.4 | 14.1 | 1.9 | ** | 16.2 | 2.3 | ** |
8MTO | 4.3 | 0.4 | 3.9 | 0.6 | 3.8 | 0.4 | ||
8MSO | 25.8 | 2.7 | 42.4 | 2.5 | ** | 55.1 | 5.3 | ** |
Total Aliphatic | 250.4 | 15.7 | 458.7 | 38.1 | ** | 528.3 | 41.2 | ** |
I3M | 52.3 | 6.6 | 63.6 | 5.5 | 65.4 | 9.2 | ||
4MO-I3M | 11.6 | 2.6 | 20.6 | 2.4 | 22 | 4 | * | |
NMO-I3M | 45.4 | 9.1 | 27.4 | 6.9 | 29.3 | 7.1 | ||
Total Indole | 109.3 | 8.3 | 111.6 | 9 | 116.7 | 11.5 |
Mean shows the average glucosinolate content in pmol per mg of tissue using the abbreviations in Table S6. SE is the standard error of the mean for that line. This data represents two independent biological replicates using 19 plants for Col-0, 31 plants containing the 35S:MYB29 transgene (16 and 15 plants from two independent transgenic lines) and 24 plants containing the 35S:MYB76 transgene (twelve plants each from two independent transgenic lines). Sig indicates the P value of the difference between Col-0 and transgenic lines containing, respectively, the 35S:MYB29 and 35S:MYB76 transgene as determined by ANOVA (Table S5). One asterisk represents a P value between 0.05 and 0.005 while two asterisks is a P value below 0.005. Cells with no asterisk represent non-significant P values, those greater than 0.05. N represents the total number of independent samples per genotypic class.