Table 3. Average Seed Glucosinolate Content in transgenic 35S:MYB lines.
Trait | Col-0 | MYB28 | MYB29 | MYB76 | |||||||
N = 8 | N = 11 | N = 11 | N = 13 | ||||||||
Mean | SE | Mean | SE | Sig | Mean | SE | Sig | Mean | SE | Sig | |
3MTP | 0.11 | 0.01 | 0.07 | 0.01 | ** | 0.20 | 0.03 | 0.14 | 0.01 | ||
3MSP | 0.12 | 0.02 | 0.46 | 0.09 | ** | 0.29 | 0.08 | 0.17 | 0.03 | ||
3OHP | 1.10 | 0.08 | 0.87 | 0.11 | 0.99 | 0.02 | 1.11 | 0.07 | |||
3BZOP | 1.56 | 0.09 | 1.59 | 0.11 | 1.83 | 0.05 | 1.75 | 0.06 | |||
4MTB | 13.63 | 1.01 | 18.91 | 1.13 | * | 17.51 | 1.07 | 16.87 | 0.63 | ||
4MSB | 1.44 | 0.17 | 6.26 | 1.61 | ** | 2.71 | 0.64 | 2.15 | 0.29 | ||
4OHB | 1.88 | 0.17 | 1.45 | 0.13 | 1.57 | 0.11 | 2.17 | 0.14 | |||
4BZOB | 1.97 | 0.06 | 1.64 | 0.18 | 1.85 | 0.14 | 2.24 | 0.08 | * | ||
5MSP | 0.10 | 0.01 | 0.71 | 0.20 | ** | 0.26 | 0.06 | ** | 0.18 | 0.01 | ** |
6MTH | 0.06 | 0.01 | 0.14 | 0.02 | ** | 0.11 | 0.02 | * | 0.12 | 0.01 | ** |
6MSH | 0.07 | 0.00 | 0.18 | 0.08 | 0.13 | 0.02 | * | 0.10 | 0.01 | ** | |
7MTH | 1.34 | 0.07 | 0.63 | 0.19 | ** | 1.46 | 0.05 | 1.45 | 0.09 | ||
7MSO | 0.52 | 0.02 | 0.55 | 0.20 | 0.71 | 0.06 | * | 0.64 | 0.04 | * | |
8MTO | 2.25 | 0.14 | 0.42 | 0.15 | ** | 2.03 | 0.11 | 2.14 | 0.14 | ||
8MSO | 4.06 | 0.17 | 2.44 | 0.67 | * | 4.60 | 0.13 | 4.73 | 0.29 | ||
Total Aliphatic | 30.97 | 1.57 | 37.99 | 3.41 | * | 37.42 | 1.85 | * | 37.01 | 1.20 | * |
I3M | 0.80 | 0.06 | 0.75 | 0.09 | 0.66 | 0.06 | * | 0.72 | 0.05 | ||
4OH-I3M | 0.01 | 0.00 | 0.03 | 0.00 | ** | 0.01 | 0.00 | 0.01 | 0.00 | ||
Total Indole | 1.05 | 0.06 | 0.95 | 0.11 | 0.86 | 0.07 | * | 0.99 | 0.05 | ||
Isoleucine | 0.74 | 0.08 | 1.53 | 0.23 | ** | 1.11 | 0.13 | 0.99 | 0.10 |
Mean shows the average glucosinolate content in nmol per mg of tissue using the abbreviations in Table S6. SE is the standard error of the mean for that line. This data represents using eight plants for Col-0, 11 plants containing the 35S:MYB28 transgene (five and six plants from two independent transgenic lines), eleven plants containing the 35S:MYB76 transgene (five and six plants each from two independent transgenic lines) and thirteen plants containing the 35S:MYB29 transgene (six and seven plants from two independent transgenic lines). Sig indicates the P value of the difference between Col-0 and transgenic lines containing the respective 35S:MYB transgene as determined by ANOVA (Table S7). One asterisk represents a P value between 0.05 and 0.005 while two asterisks is a P value below 0.005. Cells with no asterisk represent non-significant P values, those greater than 0.05. N represents the total number of independent samples per genotypic class.