Table 5. Seed glucosinolate content in T-DNA insertion mutants of MYB28, MYB29 and MYB76.
| myb28-1 | myb29 | myb76 | |||||||||||||
| WT | Homozygous | WT | Homozygous | WT | Homozygous | ||||||||||
| N = 7 | N = 11 | N = 25 | N = 17 | N = 6 | N = 9 | ||||||||||
| Mean | SE | Mean | SE | Sig | Mean | SE | Mean | SE | Sig | Mean | SE | Mean | SE | Sig | |
| 3BZOP | 0.39 | 0.07 | 0.32 | 0.04 | ** | 0.42 | 0.02 | 0.32 | 0.02 | * | 0.45 | 0.01 | 0.42 | 0.03 | |
| 4MTB | 3.5 | 0.52 | 2.73 | 0.37 | * | 3.83 | 0.18 | 3.01 | 0.21 | * | 3.53 | 0.32 | 3.86 | 0.23 | |
| 4MSB | 0.48 | 0.17 | 0.21 | 0.06 | * | 0.41 | 0.07 | 0.15 | 0.02 | 0.6 | 0.16 | 0.34 | 0.06 | * | |
| 4OHB | 0.27 | 0.04 | 0.3 | 0.04 | 0.31 | 0.02 | 0.34 | 0.03 | 0.32 | 0.05 | 0.34 | 0.03 | |||
| 4BZOB | 0.38 | 0.06 | 0.74 | 0.09 | * | 0.49 | 0.03 | 0.62 | 0.06 | ** | 0.5 | 0.04 | 0.5 | 0.03 | |
| 5MTP | 0.26 | 0.06 | 0.2 | 0.04 | * | 0.3 | 0.02 | 0.23 | 0.02 | 0.33 | 0.02 | 0.35 | 0.02 | ||
| 7MTH | 0.25 | 0.05 | 0 | 0 | ** | 0.32 | 0.02 | 0.3 | 0.04 | 0.4 | 0.01 | 0.36 | 0.03 | ||
| 7MSH | 0.2 | 0.04 | 0.01 | 0.01 | ** | 0.21 | 0.01 | 0.21 | 0.02 | 0.25 | 0.02 | 0.18 | 0.02 | * | |
| 8MTO | 0.38 | 0.06 | 0.01 | 0.01 | ** | 0.55 | 0.04 | 0.54 | 0.07 | 0.59 | 0.05 | 0.56 | 0.03 | ||
| 8MSO | 0.87 | 0.14 | 0.04 | 0.03 | ** | 1.07 | 0.06 | 0.93 | 0.1 | 1.2 | 0.05 | 1 | 0.08 | ||
| Total aliphatic | 6.86 | 1.12 | 4.52 | 0.54 | ** | 7.86 | 0.33 | 6.55 | 0.49 | * | 8.25 | 0.42 | 7.97 | 0.46 | |
| I3M | 0.18 | 0.05 | 0.28 | 0.04 | 0.21 | 0.01 | 0.22 | 0.02 | 0.25 | 0.02 | 0.22 | 0.02 | |||
Seeds are derived from homozygous or wild-type progeny of a mutant heterozygous for the myb28-1, myb29-1, myb29-2, myb76-1 and myb76-2 alleles. Mean shows the average glucosinolate content in nmol/seed using the abbreviations in Table S6. SE is the standard error of the mean for that line. This data represents two independent biological replicates, except for myb76 which only has one replicate. The data for the two myb29 alleles were pooled as there was no significant difference in the glucosinolate phenotype between the different alleles (Table S13). Sig indicates the P value of the difference between Col-0 wild-type and the homozygous mutant lines as determined by ANOVA (Table S13). One asterisk represents a P value between 0.05 and 0.005 while two asterisks is a P value below 0.005. Cells with no asterisk represent non-significant P values, those greater than 0.05. N represents the total number of independent samples per genotypic class.