FIG. 3.

TAP gene expression in response to ROFA components. BTE cells were treated with increasing doses of ROFA-leachate (A) or washed particles (B) for 6 h and then treated with or without LPS for 18 h. mRNA was isolated and analyzed by semi-quantitative RT-PCR for TAP and normalized to GAPDH. Results are expressed as fold increase over unstimulated TAP mRNA levels in the absence of ROFA components. Experiments were carried out in triplicate. In (A), the interaction between ROFA-leachate equivalent dose and LPS stimulus is significant as measured by ANCOVA (p ≤ 0.001), illustrating an increased inhibition of TAP gene expression with ROFA-leachate equivalent dose only in the presence of LPS. In (B), asterisks represent significant increase in TAP gene expression in response to washed particles (Student’s t-test, p ≤ 0.05). Error bars represent ± SEM.