Figure 3.

The proliferation of different numbers of unseparated CML-MNC, CD34⁺ CML progenitor cells, and CD34⁻ CML-MNC after 7 days of culture in progenitor cell culture medium. Proliferation was measured by [³H]thymidine incorporation (cpm) during 6 hr. Shown is the proliferation of the unseparated fraction (shaded bars), the proliferation of the CD34⁺ fraction (filled bars), and the CD34⁻ fraction (empty bars). The percentage of CD34⁺ cells was 1.9%, 6.2%, and 14.0% in patients HG, EU, and TO, respectively. In each tested concentration from each patient, the numbers of separated CD34⁺ and CD34⁻ cells tested were equivalent to the numbers of these cells in the unseparated cell fraction. All experiments were done in 6-fold.