Figure 5.

Patch–clamp recording of cells transduced with (A) AAV-CFTR in which a full length CFTR is driven by ITR, (B) AAVp5ΔC2, and (C) AAVp5D4.1C2. The durations of forskolin treatment are indicated. Downward curves indicate forskolin-activated Cl⁻ current. (D) Whole-cell current–voltage relations of each CFTR Cl⁻ channel, triangle: ITR–CFTR; open circle: AAVp5ΔC2; filled circle: AAVp5D4.1C2. Minimal conductance was detected in cells transduced with AAV–CFTR, and highest conduction was detected in cells transduced with AAVp5D4.1C2 as indicated by the slope of the I/V curve.