TABLE 1.
Primers used in this study
| Name | Sequence (from 5′ to 3′) | Target exon |
|---|---|---|
| For RACE | ||
| AmdsxF1 | CGCACAAGAGGTACTGCAAG | 2 |
| AmdsxF2 | CCTGCGAGAAGTGTAAGATCAC | 2 |
| AmdsxR1 | GACTTCTAGGTGGTTGAGGGAC | 3 |
| AmdsxR2 | CTTTATCCTGTGCCAGGTGTC | 2 |
| For RT–PCR | ||
| AmdsxF3 | CATGTCGGCTTGTCTACGCAC | 1 |
| AmdsxR3 | CGATGTAATCATTCCTCTTTGG | 7 |
| AmdsxR4 | CAAACAACCATTCGTGAAAATCTC | 5 |
| AmdsxR5 | CGCCAAGAAACCATTATGAAAC | 2 |
| AmgapdhFa | GTTCGGTGCTCAGGTTGTTG | — |
| AmgapdhRa | CCAGCTTGTAAATGACCAGAAGC | — |
a
These primers amplify the glyceraldehyde 3 phosphate dehydrogenase 1 gene used as the positive control.