TABLE 3.
ceh-39 mutations enhance XX-specific phenotypes caused by other XSE mutations
| Genotypea | Survivor phenotype | Hermaphrodite viability (%)b | nc |
|---|---|---|---|
| ceh-39(y414) | Wild type | 101 | 1008 |
| ceh-39(gk296) | Wild type | 102 | 1021 |
| ceh-39(RNAi) | Wild type | 100 | 807 |
| ceh-21(RNAi) | Wild type | 100 | 1147 |
| fox-1(y303) | Wild type | 99 | 1054 |
| sex-2(y324) | Wild type to mild Dpy | 99 | 1032 |
| sex-1(y263) | Dpy, Egl Tra | 70 | 884 |
| sex-1(RNAi) | Very Dpy, Egl, Tra | 17 | 1090 |
| sex-1(y263, RNAi) | Very Dpy, Egl, Tra | 17 | 1304 |
| sex-2(y324) sex-1(y263)d | Very Dpy | 4 | 238 |
| fox-1(y303) sex-1(y263)e | Very Dpy, Egl, Tra | 4 | 1176 |
| fox-1(y303) sex-1(RNAi) | Very Dpy, Egl, Tra | 9 | 749 |
| ceh-39(y414) sex-1(y263)f | Very Dpy, Egl, Tra | 7 | 982 |
| ceh-39(y414) sex-1(y263)e | Very Dpy, Egl, Tra | 5 | 941 |
| ceh-39(RNAi) sex-1(y263) | Very Dpy, Egl | 10 | 832 |
| ceh-39(y414) sex-1(RNAi) | Dead | 0 | 935 |
| ceh-39(gk296) sex-1(RNAi) | Dead | 0 | 1637 |
| ceh-21(RNAi) sex-1(y263) | Dpy, Egl | 75 | 1043 |
| ceh-39(y414) fox-1(RNAi) | Mild Dpy | 98 | 1486 |
| ceh-39(gk296) fox-1(RNAi) | Mild Dpy | 101 | 1256 |
| ceh-39(RNAi) fox-1(y303) | Mild Dpy | 100 | 729 |
| ceh-39(RNAi) sex-2(y324) | Dpy, Egl | 99 ± 2g | 1368 |
| fox-1(y303) sex-2(y324) | Dpy, Egl | 98 ± 1g | 1010 |
| ceh-39(RNAi) fox-1(y303) sex-2(y324) | Dpy, Egl | 92 ± 1g | 1180 |
RNAi was applied as explained in Table 1, footnote a.
Hermaphrodite viability was calculated by the following formula: (no. of adult hermaphrodites)/(total no. of embryos) × 100.
n is the total number of embryos from six independent sets of progeny counts.
Data are from C. Y. Loh and B. J. Meyer (personal communication). Of 951 progeny from sex-2(y324) sex-1(y263)/szT1 animals, only 9 (of an expected 238) lacked szT1, implying that they were sex-2(y324) sex-1(y263) and only 4% were viable. sex-2(y324) sex-1(y263) animals were severely Dpy and produced no or few progeny.
Percentage viability of fox-1(y303) sex-1(y263) XX progeny from the strain fox-1(y303) sex-1(y263)/szT1 and percentage viability of ceh-39(y414) sex-1(y263) XX progeny from the strain ceh-39(y414) sex-1(y263)/szT1 were calculated by the following formula: (no. of Dpy hermaphrodites)/0.25(n − no. of males) × 100. The szT1 balancer acts as a mild dominant him mutation, making it necessary to calculate the expected number of XX adults by subtracting the number of XO male progeny from the total number of embryos.
This strain is maintained under yEx483[dpy-30∷sdc-2(+); myo-2∷gfp(+)], an extrachromosomal array that rescues XSE mutants because it overexpresses sdc-2. To score viability, progeny from gfp(−) hermaphrodites that had lost yEx483 were counted.
Viability was calculated separately for six independent sets of progeny counts. Average viability and error are reported. Error is expressed as the standard error of the mean.