TABLE 5.
Overexpression of ceh-39 rescues XX-specific lethality caused by disruption of other XSE genes
| Genotypea | Hermaphrodite viability (%)b | nc |
|---|---|---|
| sex-1(y263) | 70 | 884 |
| sex-1(y263); yIs58[ceh-39(+)]/yIs58[ceh-39(+)]d | 98 | 1399 |
| sex-1(y263, RNAi) | 17 | 1304 |
| sex-1(y263, RNAi); yIs58/yIs58 | 71 | 597 |
| fox-1(y303) sex-1(y263) | 4 | 1176 |
| fox-1(y303) sex-1(y263); yIs58/yIs58 | 76 | 784 |
| sex-2(y324) sex-1(y263)e | 4 | 238 |
| sex-2(y324) sex-1(y263); yIs58/yIs58 | 69 | 1286 |
| ceh-39(RNAi) sex-1(y263); yIs58/yIs58f | 5 | 440 |
| ceh-39(RNAi) fox-1(y303) sex-1(y263); yIs58/yIs58f | 0 | 480 |
RNAi was applied as explained in Table 1, footnote a.
Hermaphrodite viability was calculated by the following formula: (no. of adult hermaphrodites)/(total no. of embryos) × 100.
n is the total number of embryos from six independent sets of progeny counts.
yIs58[ceh-39(+)] is an integrated transgene consisting of multiple copies of a 5.5-kbp genomic fragment spanning the ceh-39 locus.
Data are from C. Y. Loh and B. J. Meyer (personal communication). See footnote d in Table 3.
The rescue of XSE mutants is specific to increased ceh-39 dose because RNAi of ceh-39 not only abolished the suppression caused by yIs58, but also reduced the activity of ceh-39 completely, causing synergistic lethality in combination with XSE mutations.