Figure 3.—

Localization and expression of MRP-4. (A–D) Anti-MRP-4 and anti-GFP antibody staining of a 1.25-fold-stage embryo expressing the gut granule localized protein GLO-1∷GFP. White arrows denote examples of MRP-4 and GLO-1∷GFP colocalization to gut granules. Anti-MRP-4 antibody staining of wild-type (E), mrp-4(−) (F–H), and glo mutant (I–L) 1.5-fold-stage embryos is shown. In A–L intestinal cells are located between the black arrowheads. (M–O) MRP-4 does not colocalize with LMP-1∷GFP in wild-type embryos (white arrowheads). (P–R) Many (white arrows), but not all (white arrowheads), of the MRP-4 puncta colocalize with LMP-1∷GFP in intestinal cells of glo-1(−) pretzel-stage embryos. The anti-MRP-4 signal has been increased in glo-1(−) embryos relative to wild type to aid in detection and analysis. (S–Z) Anti-MRP-4 staining of a wild-type and mrp-4(cd8) adult gonads and two-cell-stage embryos. Organelles containing MRP-4 are present in oocytes (marked by brackets in S, T, W, and Z) and early-stage embryos in wild type and are lacking in mrp-4(cd8).