TABLE 1.
Analysis of birefringent gut granules
| Genotype | % of embryos lacking birefringent material in intestinal cells (n) | % of larvae lacking birefringent material in intestinal cells (n) |
|---|---|---|
| Wild typea | 0 (>100) | 0 (>100) |
| ABC transporters | ||
| mrp-4(cd8) | 93 (99) | 20 (46) |
| mrp-4(ok1095) | 96 (164) | 12 (182) |
| mrp-4(RNAi) | 93 (390) | 14 (227) |
| wht-2(RNAi) | 52 (673) | 5 (137) |
| Double mutants | ||
| mrp-4(cd8); wht-2(RNAi) | 97 (107) | 81 (89) |
| mrp-4(ok1095); wht-2(RNAi) | 100 (109) | 89 (61) |
| Mosaic RNAi | ||
| rrf-1(pk1471)a | 0 (96) | 0 (47) |
| rrf-1(pk1471); mrp-4(RNAi) | 53 (45) | 36 (22) |
| rrf-1(pk1471); wht-2(RNAi) | 3 (119) | 0 (26) |
| rsd-2(pk3307)a | 0 (84) | 0 (45) |
| rsd-2(pk3307); mrp-4(RNAi) | 0 (63) | 0 (40) |
| rsd-2(pk3307); wht-2(RNAi) | 0 (62) | 0 (24) |
| rsd-3(pk2013)a | 0 (88) | 0 (40) |
| rsd-3(pk2013); mrp-4(RNAi) | 45 (107) | 11 (28) |
| rsd-3(pk2013); wht-2(RNAi) | 0 (88) | 0 (40) |
All strains were grown at 22°. Threefold and later stage embryos or L1–L2-stage larvae were scored for the presence of birefringent material in intestinal cells using polarization microscopy. n, the number of embryos or larvae scored.
a
The same results were seen when grown on RNAi plates expressing double-stranded RNA against F33E2.4, a gene not required for the formation of birefringent gut granules.