Figure 2.—

Identification, selection, and verification of maize RFLP marker csu145-selected sorghum BAC clones. (A) Autoradiographs of Sorghum propinquum YRL filters showing six detected clones in the first filter (left) and five in the second (right). BAC a0067L02 is encircled and magnified to show one of the unique two-spot patterns from which the BAC identification is decoded. (B and C) An electrophoretic gel and autoradiograph of a blot containing the maize marker csu145, the positive control (lane 7), and the eight BACs it detected (lanes 8–15). Also included are the maize marker csu183 (lane 2), the three clones it detected (lanes 3–5), and the lambda marker (lane 1), which served as negative controls. (D) Fingerprint contig map no. 191 (http://www.genome.arizona.edu/fpc/sorghum/) showing the BACs detected by the following markers: five BACs by cdo1387 (solid circles), seven BACs by asg44 (asterisks), five BACs by csu28 (arrowheads), and eight BACs by csu145 (arrows).