Figure 3.—

An msc1 null is not synthetically sick with cnp1-1, but multicopy msc1 suppresses loss of function of CENP-A. (A) The indicated strains were grown at 25° to mid-log phase. Tenfold serial dilutions were spotted on rich media plates and incubated at the indicated temperatures for 3–4 days. (B and C) The cnp1-1 (B) or cnp1-1 mis6 (C) strain was transformed with the indicated plasmids. Cells were grown at 25° in minimal media to mid-log phase. Tenfold serial dilutions were spotted on minimal media plates and incubated at the indicated temperatures for 3–4 days.