TABLE 1.
Selected clones
| Name | Insert size (bp) | ORF or gene name | Readthrough decrease (%) |
|---|---|---|---|
| 1 | 4732 | MRPL32-YCP4-CIT2-YCR006 | −15 |
| 2 | 3490 | VPS8-SNR18-eEF1Bα | −26 |
| 3 | 5462 | MSN1-RRI2-YOL118c-MCH4 | −9 |
| 4 | 5764 | SSB1-YDL228c-HO | −46 |
| 5 | 5434 | PRC1-YMR298w-YMR299c-ADE4-ATML | −37 |
| 6 | 4572 | STB4 | −11 |
| 7 | 3945 | SUP35-ARG82-HMO1- | −25 |
| 8 | 3882 | YBR225w-YBR226c-MCX1-SLX1 | −12 |
| 9 | 4292 | RPL20B-SPS4-YOR314w-YOR314W A-5′YOR315w | −20 |
| 10 | 4092 | BNA4 -BRN1-YBL096c-YBL095w | −17 |
| 11 | 6574 | PTP1-SSB1-YDL228c-HO | −43 |
| 12 | 6033 | STU1-RIB1-HEK2-SHE1 | −13 |
| 13 | 4000 | VTS1-PDE2-PRT1 | −10 |
| 14 | 4636 | SSB1-YDL228c-HO | −35 |
| 15 | 3928 | ILV2-YMR107w-YMRWΔ15-YMRcΔ14 | −20 |
| 16 | 5429 | YAP1-GIS4-ARNtS(AGA) | −35 |
| 18 | 5140 | ATM1-PRP12 | −12 |
| 19 | 4088 | TUS1-YLRCΔ26 | −21 |
| 20 | 4531 | HFA1-ERG12-YMR209c | −5 |
| 21 | 9430 | VPS67-YKR021W-YKR022C-YKR023W-DBP7-RPC37 | −10 |
| 22 | 5170 | eEF1Bα-SNR18-VPS8 | −56 |
| 23 | 5724 | PTP1-SSB1-YDL228c-HO | −40 |
| 24 | 6257 | PIN4-SAS3-YBL053w-YBL054w | −20 |
| 25 | 3680 | SSB1-YDL228c-HO | −51 |
| 26 | 5735 | MPH2-YDL246C | −8 |
Twenty-five clones selected with the name of the genes present on the DNA fragments are listed. FS1 strain was cotransformed with pFL44L clones and pAC vector bearing a UAG stop codon. The readthrough decrease was expressed as the percentage of readthrough decrease referred to empty pFL44L vector.