TABLE 3.
Growth of the MT557/3b strain in the presence of overexpressed proteins at permissive and nonpermissive temperatures
| Generation time
|
||
|---|---|---|
| MT557/3b transformed by | 30° | 37° |
| Empty vector | 2 hr | >20 hr |
| SUP45 | 2 hr | 2 hr |
| SSB1 | 2 hr | 8 hr |
| SSB2 | 2 hr | >13 hr |
| Ssb1.435 | 2 hr | >13 hr |
| Ssb1.436 | 2 hr | >13 hr |
| Ssb1.CA | 2 hr | >13 hr |
The generation time at 30° and 37° was established from liquid culture of the MT557/3b strain in the presence or absence of overexpressed proteins Sup45p, Ssb2p, and Ssb1p and Ssb1p mutants.