Figure 7.—

UV sensitivity of ofm14, rad9Δ, and ofm14/rad9Δ strains and complementation of both the sectoring phenotype and UV sensitivity of ofm14 by pRS316RAD9. (A) Serial fivefold dilutions of the indicated strains were spotted on YPD plates. The wild-type and ofm14 strains are in the YKN10 background, the rad9Δ strain is from the yeast deletion collection (S288C-related background), and the diploid is the product of the mating of the two. The plate on the right was UV-irradiated while that on the left was not. As seen in the second row, rad9Δ fails to complement the UV sensitivity of ofm14. (B, top) Serial fivefold dilutions of the haploid wild-type, ofm14, and rad9Δ strains in the YKN10 background. Plate on the right was UV irradiated while that on the left was not. Note that the ofm14 and rad9Δ mutants show similar UV sensitivities when in the same strain background. (Bottom) Serial fivefold dilutions of the ofm14 haploid transformed with pRS316RAD9 or pRS416 alone. pRS416 is a derivative of pRS316 (Sikorski and Hieter 1989) with minor modifications in the polylinker and the lacZ gene (Brachmann et al. 1998). (C) Complementation of the ofm14 sectoring phenotype by pRS316RAD9. Representative colonies of an ofm14 strain carrying the 174-kb 5ORIΔ fragment transformed with pRS316RAD9 or pRS416 were photographed after 5 days of growth at 30° on −uracil limiting adenine plates.