TABLE 2.
Loss rates of various test chromosomes in Ofm mutants
| Loss rate per generation ± SD × 105
|
||||
|---|---|---|---|---|
| Strain | 174-kb 5ORIΔ fragmenta | 174-kb 0ORIΔ fragmentb | 142-kb 5ORIΔ fragmentc | Full-length 5ORIΔ chromosomed |
| Wild type | 210 ± 30 | 2.7 ± 1.6 | 3,800 ± 600 | 9.9 ± 3 |
| OFM1-1 | 10,000 ± 1000 | 23 ± 4 | Not recoverede | 240 ± 40 |
| ofm2 | 6,100 ± 1500 | 1,700 ± 200 | ND | ND |
| ofm5 | 5,000 ± 1000 | 930 ± 210 | ND | ND |
| ofm6 | 1,400 ± 100 | 4.2 ± 1.2 | 7,700 ± 600 | 530 ± 90 |
| ofm14 | 1,500 ± 100 | 19 ± 4 | 9,600 ± 800 | 39 ± 11 |
| rad9Δ | 2,100 ± 400 | 32 ± 6 | ND | 53 ± 9 |
ND, not determined.
a
Shown in Figure 2B; introduced by chromoduction from F510, F510-3-16, F510α4A1-4, or F510a6A.
b
Shown in Figure 2A; introduced by chromoduction from F013, F013-1-24, or F013αB2C-1C.
c
Shown in Figure 2C; introduced by chromoduction from YDN293 or YDN108.
d
Full-length chromosome deleted for ARS305, ARS306, ARS307, ARS309, and ARS310; introduced by chromoduction from YIC129.
e
Rare chromoductants were either Ura− or Trp−, indicating chromosome rearrangement, e.g., break-induced replication using the balancer chromosome to restore the sequences that had been removed by the fragmentation or had restored an origin by gene conversion.