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. 2007 Dec 13;27(1):51–61. doi: 10.1038/sj.emboj.7601958

Table 1. Kinetic constants of UNG2 and N-terminal UNG2 phosphomimicking mutants.

Enzyme Substrate Km (μM) kcat (min−1)
Without RPA
 WT U:A 1.0±0.08 449±9
 S23D U:A 1.8±0.06 870±6
 S23D, T60D U:A 1.4±0.06 641±6
 S23D, T60D, S64D U:A 1.2±0.07 726±5
       
 WT Uss 0.05±0.00 663±10
 S23D Uss 0.14±0.01 1981±13
 S23D, T60D Uss 0.09±0.01 1141±15
 S23D, T60D, S64D Uss 0.11±0.01 1360±10
       
With RPA
 WT U:A 0.59±0.09 143±5
 S23D U:A 0.70±0.06 187±7
 S23D, T60D U:A 0.66±0.06 162±7
 S23D, T60D, S64D U:A 0.67±0.07 272±6
       
 WT Uss 0.04±0.005 773±18
 S23D Uss 0.05±0.004 1204±11
 S23D, T60D Uss 0.03±0.004 546±11
 S23D, T60D, S64D Uss 0.02±0.003 882±10