Figure 2.

AP-PCR DNA fingerprints of tumor cell lines of characterized karyotypes. The cell lines (and their gender) are indicated at the top. Fingerprints were generated by using 40 and 60 ng of DNA of each cell line by using primer MCG1. The band names are at right, and the chromosomes of each band are at left. Bands G and J, from chromosomes X and 13, are double bands because the two DNA strands are resolved in these denaturing gels. Band I (chromosome 7) is polymorphic in the human population, including length polymorphisms, and there are nonlinear fluctuations in the intensity of the amplified PCR product due to sequence changes in the primer annealing regions. However, comparative analysis of tumor and normal tissues from the same individual is informative for gains or losses of these sequences because the problem of interindividual variation is eliminated. These cell lines were chosen because of their (pseudo)diploid nature (all but HT29). SW48 exhibits trisomy of 7, there is only one N13 in HuTu80 cells, and LS174-T is monosomic for X (American Tissue Cell Type Collection).