Table 1.
mef2ca mutant phenotypes resemble partial loss of edn1 function phenotypes
| % with Joint Loss in: |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| arch 1 |
arch 2 |
||||||||
| unilateral |
unilateral |
||||||||
| allele | n | bilateral | Left | Right | bilateral | Left | Right | % with BHN | % with PQP |
| b1086 | 64 | 97 | 0 | 2 | 95 | 0 | 3 | 52 | 77 |
| tn213 | 63 | 87 | 6 | 6 | 70 | 9 | 8 | 78 | 30 |
| b631 |
365 | 38 | 7 | 28 | 5 | 1 | 3 | 26 | 73 |
| mef2ca-MO: |
|||||||||
| 5 ng | 135 | 81 | 6 | 6 | 47 | 5 | 7 | 55 | 15 |
| 1.5 ng |
29 | 0 | 14 | 17 | 0 | 0 | 0 | 7 | 0 |
| edn1-MO |
|||||||||
| 0.5 ng | 105* | 23 | 3 | 7 | 32 | 4 | 10 | 21 | 21 |
Pharyngeal cartilage phenotypes seen in 4.5 dpf larvae for all three alleles of mef2ca, mef2ca morphants, and low-level edn1 morphants. Joint loss refers to absence of a joint between dorsal and ventral pharyngeal cartilages. BHN, ectopic nubbin of cartilage around basihyal; PQP, ectopic medial process emanating from palatoquadrate (see Fig. 2G-I).
*
Of 242 fish injected with 0.5 ng edn1-MO, 119 had no detectable cartilage phenotype and 18 had very severe phenotypes resembling edn1 mutant larvae. The percentages listed here are of the 105 larvae with phenotypes weaker than the edn1 mutant phenotype.