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mRNA half life determination. Cells were treated with 1 μM of Actinomycin D to block mRNA synthesis. mRNA decay was measured by detection of the 323 bp PCR product in Jurkat cells A) β-actin mRNA decay was measured by detection of 587 bp in the same cells B) The values represent the mean ± SE of (empty squares) or β-actin (filled squares) copy number per μg of total RNA from three independent experiments made by duplicate.
