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Kinetic and binding parameters for LepFNR NADPH-ferricyanide diaphorase activity
| FNR | Kd (μM, NADP+) | Km (μM, NADPH) | kcat (s-1) | kcat/Km (μM-1·s-1) |
| L. interrogans | 43 ± 3 | 19.5 ± 1.7 | 258 ± 13 | 13.2 |
| P. sativum | 32 ± 2 | 19.0 ± 1.6 | 324 ± 16 | 17.1 |
The different parameters were obtained as described in Experimental Procedures. Each parameter value represents the average of 3 independent experiments.
